nodalpoint.org - A Sanger/pyrosequencing hybrid approach for the generation of high-quality draft assemblies of marine microbial genomes, from PN - Comments http://www.nodalpoint.org/2006/07/25/a_sanger_pyrosequencing_hybrid_approach_for_the_generation_of_high_quality_draft_assemblies_of_marine_microbial_genom Comments for "A Sanger/pyrosequencing hybrid approach for the generation of high-quality draft assemblies of marine microbial genomes, from PN" en More like these to come http://www.nodalpoint.org/2006/07/25/a_sanger_pyrosequencing_hybrid_approach_for_the_generation_of_high_quality_draft_assemblies_of_marine_microbial_genom#comment-3593 <p>I think more and more people will be getting into this strategy at the beginning because 454's method much to be desired. On the other hand I think we should look carefully at how ABI is going to handle this new and emerging market. Their new high-throughput sequencer, SOLID, is based on the Agencourt sytem. </p> <p>What would be really cool is if we could get a machine that could interchange modes. Quite an instrumentation challenge because one technology is bead-based (454) - not sure about SOLID - and the other is clone based (Sanger).</p> <br class="clear" /> Fri, 11 May 2007 04:40:11 -0400 badboyz comment 3593 at http://www.nodalpoint.org A Sanger/pyrosequencing hybrid approach for the generation of high-quality draft assemblies of marine microbial genomes, from PN http://www.nodalpoint.org/2006/07/25/a_sanger_pyrosequencing_hybrid_approach_for_the_generation_of_high_quality_draft_assemblies_of_marine_microbial_genom <p>Based on the comparison of different sequencing strategies in six small marine microbial genome, the paper evaluated the utility and cost-effectiveness of a hybrid sequencing approach using 3730xl Sanger sequecing and 454 run to generate higher-quality lower-quality lower-cost assemblies compared to current Sanger sequencing strategies alone. For the genome more than 3Mb with many sequencing gaps and hard stops, the sequence strategy of 5.3X Sanger sequencing plus two 454 runs is the best choice. </p> <p>Proc Natl Acad Sci U S A. 2006 Jul 13; [Epub ahead of print] Books, LinkOut</p> <p>A Sanger/pyrosequencing hybrid approach for the generation of high-quality draft assemblies of marine microbial genomes.</p> <br class="clear" /><p><a href="http://www.nodalpoint.org/2006/07/25/a_sanger_pyrosequencing_hybrid_approach_for_the_generation_of_high_quality_draft_assemblies_of_marine_microbial_genom">read more</a></p> http://www.nodalpoint.org/2006/07/25/a_sanger_pyrosequencing_hybrid_approach_for_the_generation_of_high_quality_draft_assemblies_of_marine_microbial_genom#comments Bioinformatics Genomics Literature Sequence analysis Tue, 25 Jul 2006 23:00:40 -0400 HexiToFor 1868 at http://www.nodalpoint.org